Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cataract surgery in eyes with nanophthalmos: Operative technique and refractive outcomes - A case series.

Journal francais d'ophtalmologie·2026
Same author

[Trabecular surgeries in the management of glaucoma: A review of literature].

Journal francais d'ophtalmologie·2026
Same author

Hemorrhagic serous choroidal detachment following micropulse transscleral cyclophotocoagulation in refractory glaucoma.

Journal francais d'ophtalmologie·2026
Same author

[Imaging modalities for corneal neovascularization].

Journal francais d'ophtalmologie·2026
Same author

Real-life outcomes of the PAUL® Glaucoma Implant with ripcord in refractory glaucoma.

Journal francais d'ophtalmologie·2026
Same author

In vivo Doppler holography of the optic nerve head in glaucoma: Response to acute IOP decrease.

Journal francais d'ophtalmologie·2026

Related Experiment Video

Updated: Jul 6, 2026

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy
07:12

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Published on: September 27, 2021

[Imaging the ocular surface with confocal microscopy: histology without samples].

C Baudouin1, A Labbé, B Dupas

  • 1Service III, Centre National d'Ophtalmologie des Quinze-Vingts, Paris.

Journal Francais D'Ophtalmologie
|April 12, 2008
PubMed
Summary

New confocal microscopy offers high-resolution, painless imaging of the entire ocular surface, advancing the study of eye diseases. This technology is poised to become essential for exploring corneal and ocular surface conditions.

More Related Videos

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera
07:22

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

Published on: May 6, 2016

Related Experiment Videos

Last Updated: Jul 6, 2026

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy
07:12

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Published on: September 27, 2021

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera
07:22

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

Published on: May 6, 2016

Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Cell Biology

Context:

  • Early confocal microscopy systems (1990s) provided histological-level corneal views but were costly, difficult to use, and lacked resolution for superficial or peripheral structures.
  • These limitations restricted older confocal microscopy devices to research settings, hindering routine clinical application.
  • Recent advancements have introduced new-generation devices, such as the HRT Rostock Cornea Module, offering significant improvements.

Purpose:

  • To evaluate the capabilities of new-generation confocal microscopy for ocular surface imaging.
  • To compare the diagnostic potential of modern confocal microscopy with traditional histological methods.
  • To assess the clinical applicability of advanced confocal microscopy in diagnosing ocular surface diseases.

Summary:

  • New-generation confocal microscopy, exemplified by the HRT Rostock Cornea Module, provides high-resolution, fully digital, and minimally invasive imaging of the entire ocular surface, including the limbus and conjunctiva.
  • The image quality is comparable to ex vivo histological procedures, offering detailed visualization of corneal structures.
  • This painless technique overcomes the limitations of earlier systems, enabling comprehensive examination of superficial and peripheral ocular regions.

Impact:

  • Advanced confocal microscopy is expected to become a crucial tool for the clinical investigation of ocular surface diseases.
  • The technology's high resolution and ease of use facilitate detailed exploration of conditions affecting the cornea, limbus, and conjunctiva.
  • This minimally invasive approach promises to enhance diagnostic accuracy and patient care in ophthalmology.