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Related Experiment Video

Updated: Jul 6, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Self-pinning protein-laden drops.

Viatcheslav V Berejnov¹

¹Physics Department, Cornell University, Ithaca, NY 14853-2501, USA. berejnov@uvic.ca

Journal of Colloid and Interface Science

|April 15, 2008

Summary

Proteins in liquid drops enhance contact line pinning, significantly increasing the volume of vertically suspended drops. This pinning behavior shifts with protein concentration, affecting drop stability on surfaces.

Area of Science:

Surface science
Biophysics
Fluid dynamics

Background:

Understanding the behavior of liquid drops on solid surfaces is crucial in various scientific and industrial applications.
The interaction between proteins and surfaces can significantly alter interfacial phenomena, including drop behavior.

Purpose of the Study:

To investigate the effect of proteins on the pinning of liquid drop contact lines.
To determine how protein concentration influences drop pinning and stability on a siliconized substrate.

Main Methods:

Experiments involving drops of protein solutions on a flat siliconized substrate.
Measurement of contact angle hysteresis and surface tension of protein solutions.
Analysis of the volume of vertically pinned drops.

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Main Results:

Proteins were found to induce and enhance the pinning of the drop contact line.
This pinning effect dramatically increased the volume of vertically pinned drops.
Two distinct regimes of pinning behavior were observed based on protein concentration: increasing pinning with contact angle hysteresis at low concentrations, and decreasing pinning with decreasing surface tension at high concentrations.

Conclusions:

Protein presence fundamentally alters drop-surface interactions by enhancing contact line pinning.
The study reveals a dualistic relationship between protein concentration, surface tension, contact angle hysteresis, and drop pinning, offering insights into fluid behavior at interfaces.