Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wee1(+)-like gene in human cells.

M Igarashi1, A Nagata, S Jinno

  • 1Department of Molecular Genetics, Osaka University, Japan.

Nature
|September 5, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anterior dislocation of the shoulder : Experience with a modified clavicular harness.

International orthopaedics·2016
Same author

Diagnostic laparoscopy identifies a peritoneal adenomatoid-like mesothelioma masquerading as ovarian cancer: a case report.

European journal of gynaecological oncology·2014
Same author

Possible involvement of LKB1-AMPK signaling in non-homologous end joining.

Oncogene·2013
Same author

High-density lipoprotein cholesterol subfractions and lecithin: cholesterol acyltransferase activity in collegiate soccer players.

International journal of sports medicine·2012
Same author

Calcium phosphate mediated gene transfer into established cell lines.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

How different are the indicatrixes of the leaves of various woody plant species?

Applied optics·2010
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
See all related articles

Researchers identified a human gene, WEE1Hu, similar to the fission yeast wee1+ gene. Overexpression of WEE1Hu inhibits cell cycle progression, indicating a conserved role in cell division.

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Yeast Genetics

Background:

  • The wee1+ gene in *Schizosaccharomyces pombe* regulates the G2 to M phase transition.
  • It encodes a protein kinase with serine and tyrosine phosphorylation capabilities.

Purpose of the Study:

  • To clone and characterize a human homolog of the *S. pombe* wee1+ gene.
  • To investigate the function of the human WEE1Hu gene in cell cycle control.

Main Methods:

  • Cloning of the human WEE1Hu gene by transcomplementation of a yeast mutant.
  • Functional analysis of WEE1Hu by overexpression in fission yeast.
  • Detection of WEE1Hu messenger RNA in human cell lines.

Main Results:

Related Experiment Videos

  • The human WEE1Hu gene was cloned and found to be homologous to *S. pombe* wee1+ and mik1+ kinases.
  • Overexpression of WEE1Hu in fission yeast caused cell elongation due to G2-M transition inhibition.
  • A 3-kilobase WEE1Hu mRNA was detected in all tested human cell lines.

    • Conclusions:

    • A gene functionally similar to *wee1+* exists and is expressed in human cells.
    • WEE1Hu plays a role in regulating the G2-M cell cycle transition.