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Updated: Jul 6, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
On the relationship between GC content and the number of predicted microRNA binding sites by MicroInspector
Nicole Davis1, Natasha Biddlecom, David Hecht
1Southwestern College, 900 Otay Lakes Road, Chula Vista, CA 91910, USA.
Abstract:
MicroRNA GC content and length is believed to play a role in the prediction of putative microRNA targets. MicroInspector was evaluated to determine the extent to which these characteristics of microRNAs play a role in binding site predictive accuracy. A strong bias towards under predicting the number of expected bindings sites for low GC content sequences was observed, especially for microRNAs with <50% GC content. Researchers working with organisms with unusually low GC content should be aware of this bias.
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