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Related Experiment Video

Updated: Jul 5, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Spinophilin and the immune synapse.

Brian Seed¹, Ramnik Xavier

¹Department of Pediatrics, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, USA. bseed@ccib.mgh.harvard.edu

The Journal of Cell Biology

|April 16, 2008

Summary

Researchers observed significant cellular changes in antigen-presenting cells. Dendritic cells showed remarkable architectural polarization when interacting with T cells, revealing new insights into immune responses.

Area of Science:

Immunology
Cell Biology
Microscopy

Background:

Sensitized T cells undergo cellular organization changes when interacting with antigen-presenting cells.
Antigen-presenting cells also exhibit significant cellular alterations during T cell interactions.

Purpose of the Study:

To investigate the cellular and architectural changes in antigen-presenting cells during T cell interactions.
To visualize and characterize the dynamic cellular rearrangements in dendritic cells.

Main Methods:

Utilized a spinophilin-green fluorescent protein (GFP) fusion protein for cellular visualization.
Observed dendritic cells presenting antigens to T cells.

Main Results:

Documented dramatic changes in the cellular architecture of antigen-presenting cells.

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Imaging the Human Immunological Synapse

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Published on: March 24, 2014

Related Experiment Videos

Last Updated: Jul 5, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Published on: March 24, 2014

Captured remarkable polarization of dendritic cell structure during T cell encounters.

Conclusions:

Antigen-presenting cells undergo substantial architectural reorganization.
The study highlights significant cellular dynamics in dendritic cells during immune surveillance and response.