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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
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Spinophilin and the immune synapse

Brian Seed1, Ramnik Xavier

  • 1Department of Pediatrics, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, USA. bseed@ccib.mgh.harvard.edu

The Journal of Cell Biology
|April 16, 2008
PubMed

Extensive alterations in cellular organization are known to accompany the responses of sensitized T cells to target cells presenting an antigen of interest. Now, equally if not more dramatic changes are found to take place in cells presenting an antigen. With the help of a spinophilin-GFP fusion protein, Bloom et al. (Bloom, O., J.J. Unternaehrer, A. Jiang, J.-S. Shin, L. Delamarre, P. Allen, and I. Mellman. 2008. J. Cell Biol. 181:203-211) have captured a remarkable polarization of the cellular architecture of dendritic cells presenting an antigen to T cells.

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