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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jul 5, 2026

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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Imaging transgene activity in vivo.

Terence P F Gade1, Jason A Koutcher, William M Spees

  • 1Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Cancer Research
|April 17, 2008
PubMed
Summary

Quantitative imaging of gene therapy is crucial for clinical success. Magnetic resonance spectroscopic imaging (MRSI) now measures transgene activity in vivo, enabling precise assessment of therapeutic enzyme function and biological effects.

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Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Published on: March 15, 2013

Area of Science:

  • Biomedical Imaging
  • Gene Therapy
  • Molecular Imaging

Background:

  • Assessing transgene activity is vital for gene therapy translation.
  • Current noninvasive imaging lacks quantitative measurement of transgene action.
  • Need for in vivo methods to evaluate therapeutic transgene biological function.

Purpose of the Study:

  • To develop and apply in vivo magnetic resonance spectroscopic imaging (MRSI) for quantitative assessment of transgene activity.
  • To delineate the concentration and activity of a cytosine deaminase-uracil phosphoribosyltransferase (CD-UPRT) fusion enzyme.
  • To establish a translational imaging paradigm for preclinical and clinical gene therapy evaluation.

Main Methods:

  • In vivo magnetic resonance spectroscopic imaging (MRSI) was utilized.
  • Quantitative mapping of CD-UPRT fusion enzyme concentration and activity within tumors.
  • Correlation of imaging data with cellular CD-UPRT expression and biological effects.

Main Results:

  • MRSI successfully generated accurate maps of intratumoral fusion enzyme activity heterogeneity.
  • Excellent association observed between CD-UPRT concentration/activity and percentage of CD-UPRT(+) cells.
  • In vivo measured UPRT activity levels correlated well with the enzyme's biological impact.

Conclusions:

  • MRSI provides a precise, quantitative method for in vivo transgene activity measurement.
  • This imaging approach is a translational paradigm with potential for gene therapy applications.
  • Enables accurate assessment of therapeutic transgene function in preclinical and clinical settings.