Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid deprotection procedures for synthetic oligonucleotides.

N N Polushin1, I N Pashkova, V A Efimov

  • 1Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.

Nucleic Acids Symposium Series
|January 1, 1991
PubMed
Summary

Two new rapid methods for synthetic oligonucleotide deprotection were developed using ethanolamine or a hydrazine-ethanolamine-methanol mixture. These procedures significantly reduce deprotection time for various oligonucleotide synthesis methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Monomers containing 2'-o-alkoxymethyl groups as synthons for the synthesis of oligoribonucleotides by the phosphotriester method].

Bioorganicheskaia khimiia·2012
Same author

[Application of BODIPY-trimethylmelamine conjugate for DNA cross-linking in vitro].

Bioorganicheskaia khimiia·2011
Same author

[Methoxymethyl and (p-nitrobenzyloxy)methyl groups in the synthesis of oligoribonucleotides by the phosphotriester method].

Bioorganicheskaia khimiia·2011
Same author

Cross-linked nucleic acids: isolation, structure, and biological role.

Biochemistry. Biokhimiia·2011
Same author

[DNA mimics on the base of pyrrolidine and hydroxyproline].

Bioorganicheskaia khimiia·2011
Same author

[N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligonucleotides].

Bioorganicheskaia khimiia·2010

Area of Science:

  • Synthetic organic chemistry
  • Biochemistry
  • Molecular biology

Background:

  • Oligonucleotide synthesis is crucial for molecular biology and therapeutics.
  • Efficient deprotection is a key step in oligonucleotide synthesis.
  • Current methods can be time-consuming or require harsh conditions.

Purpose of the Study:

  • To develop rapid and efficient deprotection procedures for synthetic oligonucleotides.
  • To evaluate new reagent mixtures for oligonucleotide deprotection.
  • To optimize deprotection conditions for different oligonucleotide synthesis strategies.

Main Methods:

  • Deprotection of synthetic oligonucleotides using ethanolamine/ethanol mixtures.
  • Deprotection using pure ethanolamine.

Related Experiment Videos

  • Deprotection using a hydrazine/ethanolamine/methanol mixture.
  • Analysis of deprotection efficiency for beta-cyanoethyl phosphoramidite and H-phosphonate methods.
  • Optimization of reaction temperature and time.

    • Main Results:

    • Successful full deprotection of synthetic oligonucleotides achieved with ethanolamine-based reagents.
    • Deprotection time reduced to 30 minutes at 70°C for beta-cyanoethyl phosphoramidite and H-phosphonate methods.
    • A hydrazine-ethanolamine-methanol mixture provided rapid deprotection (12-17 min at room temperature) for specific cytosine-protected oligonucleotides.
    • The new methods are effective for oligonucleotides prepared by different synthetic routes.

    Conclusions:

    • Ethanolamine-based reagents offer a rapid and effective method for general oligonucleotide deprotection.
    • The hydrazine-ethanolamine-methanol mixture presents a highly efficient option for specific oligonucleotide deprotection protocols.
    • These advancements significantly shorten processing times in synthetic oligonucleotide preparation.