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Related Experiment Video

Updated: Jul 5, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

Variant CD44 expression by human fibroblasts.

Abirami Rajarajan1, Balvinder K Bloor, Hemini Desai

  • 1Head & Neck Cancer Research Program & Department of Oral Pathology, King's College, London, UK.

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|April 17, 2008
PubMed
Summary

Fibroblasts transcribe and express small amounts of variant exon CD44, challenging its use as a sole biomarker for cancer detection. This finding is crucial for interpreting clinical samples containing connective tissue.

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Area of Science:

  • Molecular biology
  • Cell biology
  • Cancer research

Background:

  • CD44 is a cell surface glycoprotein involved in tumor invasion and metastasis.
  • CD44 variant exon splicing is a biomarker for various carcinomas.
  • Fibroblasts are typically considered to lack CD44 variant exons, preventing false-positive signals.

Purpose of the Study:

  • To investigate the transcription and expression of CD44 variant exons (v2-v10) in fibroblasts.
  • To determine if fibroblasts contribute to CD44 variant exon expression.
  • To assess the implications of fibroblast CD44 variant exon expression on cancer biomarker analysis.

Main Methods:

  • Exon-specific reverse transcription-polymerase chain reaction (RT-PCR) for variant exons v2-v10.
  • Analysis of normal primary, immortalized, and experimentally transformed fibroblasts.

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Last Updated: Jul 5, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

Murine Dermal Fibroblast Isolation by FACS
06:04

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
11:30

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

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  • Flow cytometry, immunocytochemistry, and Western blotting for expression analysis.

    • Main Results:

    • All fibroblast types transcribed low levels of variant exon mRNA.
    • Minor expression of some variant exons was detected by flow cytometry in cultured fibroblasts.
    • Expression was not detectable by Western blotting or immunocytochemistry.

    Conclusions:

    • Fibroblasts transcribe and express small amounts of variant exon CD44.
    • The presence of variant exon CD44 in fibroblasts may lead to false-positive signals.
    • This finding necessitates consideration of fibroblast contribution when using CD44 splicing as a malignancy biomarker in clinical samples with connective tissue.