Related Experiment Video
Updated: Jul 5, 2026

Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
[Dynamics of phosphate mobilization by Enterobacter dissolvens and Pseudomonas putida strains from tricalcium
Abstract:
High phosphate-mobilizing activity of Pseudomonas putida and Enterobacter dissolvens strains was determined when investigating the dynamics of Ca3(PO4)2 mobilization in the system bacterial cell--medium. P. putida in 5 days and E. dissolvens in one day of cultivation dissolved 66 and 57%, respectively, in the medium with 5 g/l primary concentration of Ca3(PO4)2. There are differences in processes of phosphorus mobilization between these bacterial strains. Maximal concentration of soluble phosphorus (12.2 mmol) in the medium where E. dissolvens was cultivated was accompanied by pH decrease from 7.0 to 4.1. In case of P. putida the process of solubilization proceeded in conditions of decreasing pH (7.0-4.9), and when pH increased (pH 4.9-5.6). These data may evidence for different mechanisms of Ca3(PO4)2 mobilization in these twvo bacteria strains.
Related Concept Videos
The Phosphorus Cycle
Roles of Electrolytes: Calcium and Phosphate
The calcium concentration in blood plasma is primarily regulated...
Factors Affecting Solubility
Microbial Bioremediation of Uranium
ATP Driven Pumps I: An Overview
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
Introduction to Electrolytes
Role of Sodium
One...

