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Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

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Related Experiment Video

Updated: Jul 5, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Wnt signaling and adipocyte lineage commitment.

Robert R Bowers¹, M Daniel Lane

¹Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA.

Cell Cycle (Georgetown, Tex.)

|April 18, 2008

Summary

This summary is machine-generated.

The Wnt signaling pathway is activated early in fat cell development. This research reveals key gene changes during the commitment of mesenchymal stem cells (MSCs) to preadipocytes, offering insights into obesity mechanisms.

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Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Area of Science:

Cell Biology
Molecular Biology
Stem Cell Research

Background:

Obesity involves increased mature fat cells derived from preadipocytes, originating from mesenchymal stem cells (MSCs).
Mechanisms controlling MSC commitment to preadipocytes, an early adipogenesis event, are poorly understood.

Purpose of the Study:

Investigate early molecular events in adipogenesis, specifically MSC commitment to preadipocytes.
Identify differentially expressed genes during this critical transition using a cell model.

Main Methods:

Utilized C3H10T1/2 cells as a MSC model and A33 cells as a preadipocyte model.
Employed microarray technology and quantitative real-time RT-PCR to analyze gene expression.
Examined Wnt signaling pathway components, including R-spondins and beta-catenin.

Main Results:

Microarray and RT-PCR revealed differential gene expression in Wnt signaling between MSCs and preadipocytes.
R-spondins-2 and -3 were significantly upregulated in proliferating preadipocytes.
Beta-catenin nuclear accumulation was observed in preadipocytes, indicating Wnt pathway activation.

Conclusions:

Activation of the Wnt signaling pathway is an early and critical event in adipogenesis.
Differential expression of Wnt pathway genes, including R-spondins and Lef/Tcf factors, marks MSC commitment to preadipocytes.