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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Clinical features and molecular diagnosis of Chikungunya fever from South India

Vemu Lakshmi1, Mamidi Neeraja, M V S Subbalaxmi

  • 1Department of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, India. lgorthi@hotmail.com

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
|April 19, 2008
PubMed

Insights

Chikungunya fever re-emerged in India in 2006, causing severe rheumatic symptoms. A rapid, cost-effective molecular test, real-time loop-mediated isothermal amplification (RT LAMP), aided in diagnosing this vectorborne viral illness.

Area of Science:

  • Virology
  • Epidemiology
  • Molecular Diagnostics

Background:

  • Chikungunya fever, a vectorborne viral illness, resurged in India in 2006 after a 33-year absence.
  • The epidemic was characterized by fever, rash, and severe rheumatic manifestations.

Purpose of the Study:

  • To highlight the clinical features of Chikungunya virus infection.
  • To evaluate molecular diagnostic methods for Chikungunya virus.
  • To identify the etiological agent of the 2006 Indian epidemic.

Main Methods:

  • Clinical observation of infected individuals.
  • Molecular diagnostic techniques including reverse-transcriptase polymerase chain reaction (RT-PCR).
  • Development and application of real-time loop-mediated isothermal amplification (RT LAMP) assay.
  • Isolation and genotyping of Chikungunya virus from patient samples.

Main Results:

  • The East Central South African genotype of Chikungunya virus was identified as the cause of the epidemic.
  • RT LAMP detected 20 more positive cases than RT-PCR.
  • RT LAMP proved to be a rapid, cost-effective diagnostic tool suitable for resource-limited settings.

Conclusions:

  • Molecular diagnostics are crucial for identifying emerging vectorborne viral diseases like Chikungunya.
  • RT LAMP offers a valuable alternative for rapid and accessible diagnosis of Chikungunya virus infection.

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