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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Published on: June 28, 2018
A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains
Laura Steindler1, Giulia Devescovi, Sujatha Subramoni
1Bacteriology Group, International Centre for Genetic Engineering & Biotechnology, Trieste, Italy.
Abstract:
Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.
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