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Updated: Jul 5, 2026
Mouse Adipose Tissue Collection and Processing for RNA Analysis
Published on: January 31, 2018
[Study on SOCS-3, PPARgamma and ACO mRNA expression of adipose tissues in obese rats with leptin resistance]
1School of Public Health, China Medical University, Shenyang 110001, China. liuli@mail.cmu.edu.cn
Objective:
To study the difference of socs-3, PPARgamma and ACO mRNA expression in obese rats with leptin resistance induced by high-fat diet.
Methods:
6 Wistar male rats were fed with basic diet as control, and 24 rats were fed with high-fat diet as high-fat diet group. At the end of the 8th week, 8 obese rats were selected by body weight from high-fat diet group as obese group. Serum leptin, socs-3, PPARgamma and ACO mRNA were detected.
Results:
In compare with control group, body weight, serum leptin, socs-3 and PPARgamma mRNA expressions of epididymal adipose tissues were significantly increased in obese group, while ACO mRNA were markedly decreased.
Conclusion:
Obese rats induced by high-fat diet showed the suppression of leptin signaling transduction pathway, decreases in fatty acid oxidation and increases in fat synthesis.
