[Application of fingerprint of active part on extraction techniques for Vernonia anthelmintica]
Abstract
Objective:
To probe into feasibility of extraction techniques for Vernonia anthelmintica using HPLC fingerprint of active part as model.
Methods:
The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices.
Results:
The optimum extracting procedure was as follows: using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each.
Conclusion:
It is scientific, simple and applicable.
