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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Updated: Jul 5, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Electrochemical immunosensor with aptamer-based enzymatic amplification.

Kejun Feng¹, Yan Kang, Jing-Jin Zhao

¹State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.

Analytical Biochemistry

|April 22, 2008

Summary

This summary is machine-generated.

This study presents a novel electrochemical immunosensor for detecting immunoglobulin E (IgE). The aptamer-based system offers high specificity and sensitivity for IgE detection, paving the way for advanced diagnostics.

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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

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Published on: October 23, 2009

Area of Science:

Electrochemistry
Biosensors
Immunotechnology

Background:

Electrochemical immunosensors are crucial for detecting biomarkers.
Immunoglobulin E (IgE) is a key indicator in allergic diseases.
Aptamer-based amplification offers enhanced sensitivity in biosensing.

Purpose of the Study:

To develop and validate an electrochemical immunosensor for IgE detection.
To utilize aptamer-based enzymatic amplification for signal enhancement.
To achieve high sensitivity and specificity in IgE quantification.

Main Methods:

Covalent immobilization of IgE antibody on a gold electrode.
Aptamer-based enzymatic amplification using streptavidin-conjugated alkaline phosphatase.
Enzymatic reduction of silver ions to metallic silver for signal generation.
Quantification via stripping voltammetry.

Main Results:

The developed immunosensor demonstrated high specificity for IgE.
A wide dynamic range was achieved for IgE detection.
A low detection limit was observed, attributed to aptamer specificity and silver deposition amplification.
Successful quantification of IgE was achieved using stripping voltammetry.

Conclusions:

The aptamer-based electrochemical immunosensor is a sensitive and specific platform for IgE detection.
Enzymatic amplification of deposited silver significantly enhances detection limits.
This approach holds promise for developing advanced diagnostic tools for IgE-related conditions.