[Agrobacterium tumefaciens-mediated transformation of the pathogen of Botrytis cinerea]
Wei-Feng Shen1, Hong-Biao Weng, Bao-Long Niu
1Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China. weifsh@126.com
Yi Chuan = Hereditas
|April 22, 2008
Abstract:
Employing gfp as a reporter gene and hygromycin gene (hph) as a selection marker, the recombinant vector pKPG was constructed and transformed into fresh conidia of Botrytis cinerea via Agrobacterium tumefaciens. Transformants were identified by PCR analysis of gfp and hph cassette, green fluorescence observation with microscope and Southern hybridization. Results confirmed that target genes were successfully integrated into the genome of Botrytis cinerea.
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