Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stabilizing method for reflection interference contrast microscopy.

Kipom Kim1, Omar A Saleh

  • 1Materials Department, University of California, Santa Barbara, Santa Barbara, California 93106, USA.

Applied Optics
|April 22, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing an integrated brain resource framework for translational neuroscience in Korea Brain Bank.

Frontiers in neurology·2026
Same author

Diffusive and Enzymatic Modulation of the Dynamic Size Distribution of DNA Droplets.

Physical review letters·2026
Same author

Diverse, distinct, and densely packed DNA nanostar droplets.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Fibrinolysis for Apical Thrombus as a Bridge to Emergent Valve-in-Valve Transcatheter Aortic Valve Replacement.

JACC. Case reports·2025
Same author

Condensation and activator/repressor control of a transcription-regulated biomolecular liquid.

Soft matter·2025
Same author

Cooperation and Competition of Base Pairing and Electrostatic Interactions in Mixtures of DNA Nanostars and Polylysine.

Journal of the American Chemical Society·2025
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Reflection interference contrast microscopy (RICM) requires stable focus for long acquisitions. A new image-analysis feedback method minimizes drift, achieving 0.12 nm height stability for beads over 45 minutes.

Area of Science:

  • Biophysics
  • Microscopy techniques
  • Surface science

Background:

  • Reflection interference contrast microscopy (RICM) is vital for measuring microscopic object shape and position in solution.
  • Long-time RICM acquisitions are challenged by defocusing effects from thermal and mechanical drift.

Purpose of the Study:

  • To present a simple method for stabilizing RICM.
  • To minimize defocusing effects during long-time acquisitions.

Main Methods:

  • Implemented an image-analysis-based linear focus function.
  • Established feedback control of the focal position.
  • Used RICM for independent measurement of immobilized bead vertical position fluctuations.

Main Results:

Related Experiment Videos

  • Achieved high stability with a standard deviation of 0.12 nm in measured height.
  • Demonstrated stability during a 45-minute acquisition under feedback control.

Conclusions:

  • The presented stabilizing method effectively minimizes drift in RICM.
  • This approach enables high-stability, long-time acquisitions for biophysical and biological applications.