Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remote sensing data and machine learning models estimate sorghum grain yield in a plant breeding program.

Plant phenomics (Washington, D.C.)·2026
Same author

Safety, immunogenicity, and dose ranging of a bivalent respiratory syncytial virus and human metapneumovirus glycoprotein F-molecular clamp vaccine candidate in older adults: 1-month interim analysis of an ongoing randomised, observer-blind, placebo- and active-controlled, phase 1 trial.

Vaccine·2026
Same author

Stepwise loss of complexity in hagfish eyes prior to deep sea colonization.

Biology letters·2026
Same author

Viral mimicry redirects immunosuppressed colorectal tumour landscapes towards a proinflammatory and CMS1-like regenerative state.

Communications biology·2026
Same author

Physiological and Yield Responses of Peanut (<i>Arachis hypogaea</i> L.) Genotypes Under Well-Watered and Water-Stressed Conditions.

Plants (Basel, Switzerland)·2026
Same author

Nerve Combing for Idiopathic Trigeminal Neuralgia Without Neurovascular Compression.

Journal of clinical and experimental dentistry·2026

Related Experiment Video

Updated: Jul 5, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Protein trafficking into autophagosomes.

Andrew Young1, Sharon Tooze

  • 1Cancer Research UK, London Research Institute, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2008
PubMed
Summary

New methods identify the location of secretory proteins within the cell, aiding research into autophagy and autophagosome formation. This helps understand how cellular compartments contribute to this vital process.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Secretory proteins traffic through various intracellular compartments.
  • The trans-Golgi network (TGN) and endosomes are key linked compartments involved in protein trafficking.
  • The precise role of these compartments in autophagosome formation during autophagy is not well understood.

Purpose of the Study:

  • To develop and describe methods for assigning intracellular localization of secretory proteins.
  • To investigate the role of the trans-Golgi network (TGN) and endosomes in autophagy.
  • To advance the understanding of autophagosome biogenesis.

Main Methods:

  • Development of novel techniques to determine the subcellular localization of soluble and membrane-associated secretory proteins.

More Related Videos

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

Related Experiment Videos

Last Updated: Jul 5, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

  • Utilizing protein trafficking studies between the TGN and endosomes.
  • Investigating the connection between these compartments and autophagosome formation.

    • Main Results:

    • Established methods for precise intracellular localization of secretory proteins.
    • Highlighted the interconnectedness of the TGN and endosomes in protein transport.
    • Provided a foundation for further research into the role of these compartments in autophagy.

    Conclusions:

    • The described methods enable accurate assignment of secretory protein localization to specific compartments like the TGN and endosomes.
    • These compartments play a significant role in the complex process of autophagosome formation.
    • Further investigation is crucial to elucidate the detailed mechanisms of autophagy.