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Updated: Jul 5, 2026

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
[Analysis on expression and molecular basis for ABO glycosyltransferase with dual specificity]
Zhi-Hui Deng1, Hao Zhang, Jian-Qiang Zeng
1Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China. zhihui_deng@yahoo.com.cn
Abstract:
In order to elucidate the expression and molecular genetic background of ABO gene seven samples with ABO discrepancy further identified as bi-specific ABO gene were studied. All these samples were subjected to phenotyping by monoclonal and polyclonal antisera and were then genotyped by direct DNA sequencing and haplotype-sequencing at the exon 6 and 7 of ABO gene. As a result, six ABO dual-specific alleles were identified in Chinese population. An antigen expressed by these B (A) or Cis-AB individuals varied from very low level to the normal level, compared with common A blood group samples. In conclusion, molecular genetic backgrounds of two pairs out of four samples in all samples were the same, however, the ABO expression showed diverse.
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