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Related Concept Videos

Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

DcpS scavenger decapping enzyme can modulate pre-mRNA splicing.

Vincent Shen1, Hudan Liu, Shin-Wu Liu

  • 1Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854-8082, USA.

RNA (New York, N.Y.)
|April 23, 2008
PubMed
Summary

The scavenger decapping enzyme DcpS, previously known for mRNA decay, also impacts nuclear pre-mRNA splicing. It shuttles between the nucleus and cytoplasm, affecting splicing by interacting with cap-binding proteins like Cbp20.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • RNA Metabolism
  • Gene Expression Regulation

Background:

  • The scavenger decapping enzyme (DcpS) is primarily known for its role in cytoplasmic mRNA decay.
  • Immunofluorescence studies unexpectedly localized DcpS to the nucleus, suggesting a broader function.

Purpose of the Study:

  • To investigate the nucleocytoplasmic localization and function of DcpS.
  • To determine if DcpS influences nuclear RNA processing events beyond mRNA decay.

Main Methods:

  • Characterization of DcpS as a nucleocytoplasmic shuttling protein using import/export signals.
  • Generation of a DcpS-depleted cell line via shRNA to assess functional consequences.
  • Analysis of pre-mRNA splicing efficiency using reporter minigenes and endogenous genes.
  • In vitro assays to examine the interaction between DcpS and cap-binding proteins, specifically Cbp20.

Main Results:

  • DcpS shuttles between the nucleus and cytoplasm, possessing distinct nuclear import and export signals.
  • Reduced DcpS levels significantly impaired cap-proximal intron splicing of both reporter and endogenous genes.
  • DcpS was shown to displace Cbp20 from the cap structure, indicating a mechanism for sequestering cap-binding proteins.
  • Complementation with Cbp20 restored normal splicing, confirming DcpS's role in splicing modulation via Cbp20.

Conclusions:

  • DcpS has a novel function in nuclear pre-mRNA splicing, extending its known role in mRNA decay.
  • DcpS's nucleocytoplasmic shuttling and cap-binding activity influence RNA processing in the nucleus.
  • The findings reveal a broader significance of DcpS in regulating gene expression through its involvement in both mRNA decay and pre-mRNA splicing.