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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jul 5, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Colony hybridization to screen for microsatellites.

T J Hudson1

  • 1Whitehead Institute, Cambridge, Massachusetts, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary

Simple sequence length polymorphisms (SSLPs) are genetic markers derived from microsatellite DNA. This study details a rapid, optimized method for identifying these repetitive DNA sequences using colony hybridization and labeled probes.

Area of Science:

  • Genomics
  • Molecular Biology

Background:

  • Simple sequence length polymorphisms (SSLPs) are valuable genetic markers.
  • Microsatellite repeat sequences are abundant in eukaryotic genomes.

Purpose of the Study:

  • To describe an optimized protocol for identifying microsatellite DNA.
  • To facilitate the rapid processing of genetic marker identification.

Main Methods:

  • Colony hybridization using oligonucleotide probes for simple sequence repeat (SSR) DNA.
  • Transfer of plasmid or phagemid colonies to nylon filters.
  • Hybridization with 32P-labeled oligonucleotide probes.

Main Results:

  • The protocol is adapted for rapid, high-throughput processing of multiple filters.

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Last Updated: Jul 5, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
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Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

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  • Optimization focuses on identifying long stretches of uninterrupted repetitive DNA sequences.

    • Conclusions:

    • This method enables efficient identification of microsatellite DNA for genetic marker development.
    • The optimized protocol enhances the speed and accuracy of detecting repetitive DNA sequences.