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Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
Published on: August 6, 2014
ENU mutagenesis in the mouse
J David McDonald1, David Beier
1Wichita State University, Wichita, Kansas, USA.
Current Protocols in Human Genetics
|April 23, 2008
Abstract:
This unit describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to achieve very highly induced rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.
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