Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC).

Bing Yu1, Nicole A Sawyer, Christine Chiu

  • 1Department of Molecular and Clinical Genetics, Central Clinical School and SUPAMAC The University of Sydney, New South Wales, Australia.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Light-assisted preparation of vancomycin chiral stationary phase based on diazotized silica and its enantioseparation evaluation by high-performance liquid chromatography.

Talanta·2018
Same author

Novel Adipokine, FAM19A5, Inhibits Neointima Formation After Injury Through Sphingosine-1-Phosphate Receptor 2.

Circulation·2018
Same author

Liquid-Crystalline Dynamic Networks Doped with Gold Nanorods Showing Enhanced Photocontrol of Actuation.

Advanced materials (Deerfield Beach, Fla.)·2018
Same author

KCa(H<sub>2</sub> O)<sub>2</sub> [Fe<sup>III</sup> (CN)<sub>6</sub> ]⋅H<sub>2</sub> O Nanoparticles as an Antimicrobial Agent against Staphylococcus aureus.

Angewandte Chemie (International ed. in English)·2018
Same author

Warming deferentially altered multidimensional soil legacy induced by past land use history.

Scientific reports·2018
Same author

Advanced Modified Polyacrylonitrile Membrane with Enhanced Adsorption Property for Heavy Metal Ions.

Scientific reports·2018
Same journal

Resolving Breakpoints of Chromosomal Rearrangements at the Nucleotide Level Using Sanger Sequencing.

Current protocols in human genetics·2020
Same journal

Informed Consent for Genetic and Genomic Research.

Current protocols in human genetics·2020
Same journal

A Guide to Using ClinTAD for Interpretation of DNA Copy Number Variants in the Context of Topologically Associated Domains.

Current protocols in human genetics·2020
Same journal

The AD Knowledge Portal: A Repository for Multi-Omic Data on Alzheimer's Disease and Aging.

Current protocols in human genetics·2020
Same journal

A Practical Guide for Structural Variation Detection in the Human Genome.

Current protocols in human genetics·2020
Same journal

Identification and Genotyping of Transposable Element Insertions From Genome Sequencing Data.

Current protocols in human genetics·2020
See all related articles

Denaturing High-Performance Liquid Chromatography (DHPLC) efficiently detects DNA mutations like single-base substitutions and small insertions/deletions. This automated method aids in high-throughput mutation detection and SNP discovery for genetic research.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Candidate gene scanning is crucial for identifying genetic variations.
  • High-throughput mutation detection methods are needed for genetic research.
  • Single-nucleotide polymorphism (SNP) discovery requires efficient screening tools.

Purpose of the Study:

  • To evaluate Denaturing High-Performance Liquid Chromatography (DHPLC) as an efficient method for candidate gene scanning.
  • To assess the capability of DHPLC in detecting various DNA mutations.
  • To highlight DHPLC's utility in high-throughput genetic analysis and SNP discovery.

Main Methods:

  • Utilizing Denaturing High-Performance Liquid Chromatography (DHPLC) for DNA analysis.
  • Exploiting differential retention of homoduplex and heteroduplex DNA species.

Related Experiment Videos

Last Updated: Jul 5, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

  • Employing partial thermal denaturation conditions for DNA fragment analysis.

    • Main Results:

    • DHPLC demonstrated high efficiency and automation for candidate gene scanning.
    • The method successfully detected single-base substitutions.
    • Insertions or deletions up to 1.5 kb in PCR amplified DNA fragments were identified.

    Conclusions:

    • DHPLC is an effective platform for automated, high-throughput mutation detection.
    • The method facilitates efficient SNP discovery.
    • DHPLC offers a robust solution for analyzing genetic variations in DNA fragments.