Related Experiment Video
Updated: Jul 5, 2026
06:04
An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios
Published on: December 8, 2023
Optimization of transfection
1Yale University School of Medicine, New Haven, Connecticut, USA.
Current Protocols in Neuroscience
|April 23, 2008
Abstract:
The single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate-mediated gene transfer, DEAE-dextran-mediated gene transfer, electroporation, and liposome-mediated transfection. Fusion techniques such as protoplast fusion and microinjection may also be considered.
