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Related Concept Videos

Rabies01:28

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...
Neural Circuits01:25

Neural Circuits

Neural circuits and neuronal pools are two of the main structures found in the nervous system. Neural circuits are networks of neurons that work together to carry out a specific task or process. They consist of interconnected neurons and glial cells, which provide structural and metabolic support.
Neuronal pools are collections of nerve cells with similar functions and interact through chemical and electrical signals. These pools include both interneurons (the central neural circuit nodes that...

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Related Experiment Video

Updated: Jul 5, 2026

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
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Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Published on: September 14, 2015

Transneuronal circuit analysis with pseudorabies viruses.

J P Card1, L W Enquist

  • 1University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary

This study details using pseudorabies virus (PRV) as a transneuronal tracer for mapping neuronal circuits. Protocols cover experimental design, viral stock preparation, and immunohistochemistry for circuit analysis.

Area of Science:

  • Neuroscience
  • Virology

Background:

  • Viruses are increasingly used as transneuronal tracers to map neuronal circuitry.
  • This method relies on neurotropic viruses infecting neurons and spreading to connected neurons via synapses.

Purpose of the Study:

  • To present protocols for using pseudorabies virus (PRV) for polysynaptic analysis of neuronal circuits.
  • To detail experimental design aspects crucial for successful viral tracing in circuit definition.

Main Methods:

  • Utilizing swine alpha herpesvirus, pseudorabies virus (PRV), for transneuronal tracing.
  • Describing retrograde infection of CNS circuits via peripheral injection in rats.
  • Detailing transneuronal analysis through intracerebral injection and with multiple recombinant strains.
  • Including methods for viral stock preparation, titration, and immunohistochemical localization of viral antigens.

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Related Experiment Videos

Last Updated: Jul 5, 2026

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
13:12

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Published on: September 14, 2015

Viral Tracing of Genetically Defined Neural Circuitry
13:06

Viral Tracing of Genetically Defined Neural Circuitry

Published on: October 17, 2012

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
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Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

Main Results:

  • Established protocols for PRV-based transneuronal tracing in rat CNS.
  • Demonstrated application in both retrograde and intracerebral tracing strategies.
  • Provided comprehensive methods for viral stock management and antigen detection.

Conclusions:

  • PRV is an effective tool for polysynaptic analysis and neuronal circuit definition.
  • The presented protocols complement classical tract tracing methods.
  • Successful implementation requires careful experimental design and specific viral handling techniques.