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Updated: Jul 5, 2026

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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
Published on: February 18, 2020
Cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH)
1University of Arizona, Tucson, Arizona, USA.
Current Protocols in Neuroscience
|April 23, 2008
Abstract:
This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.

