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Related Experiment Video

Updated: Jul 5, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
08:57

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Protein expression in the baculovirus system.

A Bernard1, M Payton, K R Radford

  • 1Ares-Serono Pharmaceutical Research Institute, Geneva, Switzerland.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

This study details large-scale recombinant protein production using insect cell-baculovirus systems. It provides optimized methods for viral stock production, titration, and scaled-up bioreactor cultivation for efficient protein expression.

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Last Updated: Jul 5, 2026

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Expression Systems

Background:

  • Insect cell-recombinant baculovirus co-cultures provide soluble recombinant proteins with post-translational modifications.
  • The large baculovirus genome facilitates cloning large DNA segments for complex protein aggregate expression.

Purpose of the Study:

  • To describe methods for the large-scale production of recombinant proteins utilizing the baculovirus expression system.
  • To provide protocols for viral stock preparation, titration, and optimization of expression conditions.
  • To outline techniques for large-scale bioreactor cultivation and protein harvesting.

Main Methods:

  • Large-scale viral stock production and titration (plaque and end-point assays).
  • Small-scale culture testing to determine expression kinetics and optimize conditions (cell lines, media, parameters).
  • Large-scale production in fermentors using batch or fed-batch procedures in perfusion cultures.

Main Results:

  • Established protocols for preparing and titering viral stocks.
  • Identified optimal cell culture and infection conditions for enhanced protein production.
  • Developed methods for large-scale bioreactor production and harvesting of recombinant proteins.

Conclusions:

  • The baculovirus expression system offers a robust platform for large-scale recombinant protein production.
  • Optimized protocols ensure efficient production of soluble proteins with desired modifications.
  • This unit provides a comprehensive guide for scalable protein manufacturing using insect cells and baculovirus.