Microdialysis in rodents
A C Thompson1, T S Shippenberg
1NIH/NIDA Intramural Research Program, Baltimore, Maryland, USA.
Current Protocols in Neuroscience
|April 23, 2008
Summary
Microdialysis is a key in vivo sampling method for measuring substances in blood and tissue. This technique utilizes a semipermeable membrane for substance analysis and infusion in research settings.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Pharmacology
Background:
- Microdialysis is a well-established technique for in vivo substance measurement in biological fluids and tissues.
- It enables the quantification of various molecules, including neurotransmitters, hormones, and pharmaceuticals.
- Recent advancements allow for the infusion of substances into the central nervous system.
Purpose of the Study:
- To describe the construction of different microdialysis probe designs (concentric, side-by-side, horizontal).
- To detail the surgical implantation and in vitro/in vivo application of microdialysis in rodents.
- To provide methods for quantitative microdialysis.
Main Methods:
- Utilizing a semipermeable dialysis membrane perfused with physiological fluid.
- Employing passive diffusion for molecule migration across the membrane based on concentration gradients.
- Collecting diffused molecules for subsequent quantification.
Main Results:
- Construction methods for concentric, side-by-side, and modified horizontal microdialysis probes are detailed.
- Surgical procedures for probe implantation in rats and mice are outlined.
- Protocols for in vitro and in vivo microdialysis in rodents are provided.
Conclusions:
- This unit provides a comprehensive guide to microdialysis probe construction and application.
- It facilitates in vivo and in vitro substance analysis and infusion in rodent models.
- The described quantitative techniques enhance the utility of microdialysis in research.
