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Microdialysis in nonhuman primates.

R C Saunders1, B S Kolachana, D R Weinberger

  • 1NIMH/Clinical Brain Disorders Branch, Bethesda, Maryland, USA.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary
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This study details a reproducible method for microdialysis in sedated or awake monkeys. The technique enables repeated in vivo sampling for neurochemical analysis in non-human primates.

Area of Science:

  • Neuroscience
  • Primate Research
  • Analytical Chemistry

Background:

  • Microdialysis is a valuable technique for in vivo neurochemical analysis.
  • Previous methods for microdialysis in non-human primates were limited in reproducibility and applicability.
  • A standardized protocol is needed for routine microdialysis in monkeys.

Purpose of the Study:

  • To present a standardized technique for routine repeated microdialysis experiments in monkeys.
  • To provide detailed protocols for both sedated and awake, behaving preparations.
  • To facilitate in vivo neurochemical analysis in primate models.

Main Methods:

  • Development of protocols for dialysis probe construction and in vitro calibration.
  • Utilizing magnetic resonance imaging (MRI) for pre- and post-operative anatomical verification.

Related Experiment Videos

  • Surgical implantation of a guide holder for probe placement in the monkey brain.
  • In vivo microdialysis sample collection and subsequent neurochemical analysis.
  • Training protocols for awake, behaving monkeys to ensure minimal disturbance during experiments.

    • Main Results:

    • Successful implementation of a technique for routine repeated microdialysis in monkeys.
    • Demonstration of feasibility in both sedated and awake, behaving animal models.
    • Accurate anatomical verification of probe placement using MRI.
    • Collection of samples for comprehensive neurochemical analysis.

    Conclusions:

    • The presented technique allows for routine and reproducible microdialysis in monkeys.
    • This method is adaptable for both sedated and awake preparations, broadening its applicability.
    • The protocol facilitates advanced neurochemical studies in primate models, aiding neuroscience research.