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Related Experiment Video

Updated: Jul 5, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Published on: January 29, 2019

Multidisciplinary approaches for characterizing synaptic vesicle proteins.

Miriam Leenders¹, Claudia Gerwin, Zu-Hang Sheng

¹National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This summary is machine-generated.

This study details methods to identify and characterize synaptic vesicle proteins, crucial for understanding neurotransmission and synaptic vesicle exocytosis molecular mechanisms.

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Last Updated: Jul 5, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

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Published on: January 29, 2019

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Published on: September 3, 2014

Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

Synaptic vesicle proteins are key to neurotransmission.
Understanding their molecular mechanisms is vital for neuroscience research.

Purpose of the Study:

To present multidisciplinary approaches for identifying and characterizing synaptic vesicle proteins.
To advance knowledge of molecular mechanisms in synaptic vesicle exocytosis and neurotransmission.

Main Methods:

Subcellular fractionation of rat brain synaptosomes to isolate synaptic vesicles.
Pre-embedding immunogold staining and electron microscopy for morphological identification.
Immunocytochemical staining of cultured hippocampal neurons for light microscopy.

Main Results:

Established protocols for isolating synaptic vesicles.
Enabled morphological identification of synaptic vesicle proteins in situ.
Facilitated localization studies of synaptic vesicle proteins in cultured neurons.

Conclusions:

Multidisciplinary approaches effectively identify and characterize synaptic vesicle proteins.
These methods enhance understanding of synaptic vesicle exocytosis and neurotransmission.
The presented protocols support diverse biochemical and imaging studies.