Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Patch-clamp recording from neuronal dendrites.

Nicholas P Poolos¹, Terrance D Jones

¹University of Washington, Seattle, Washington, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sex-specific proteomic analysis of epileptic brain tissues from Pten knockout mice and human refractory epilepsy.

Experimental neurology·2025

Same author

Sex-specific proteomic analysis of epileptic brain tissues from Pten knockout mice and human refractory epilepsy.

bioRxiv : the preprint server for biology·2025

Same author

Drug-resistant epilepsy associated with peripheral complement decreases and sex-specific cytokine imbalances: a pilot study.

Scientific reports·2025

Same author

Sex-Specific Complement and Cytokine Imbalances in Drug-Resistant Epilepsy: Biomarkers of Immune Vulnerability.

bioRxiv : the preprint server for biology·2024

Same author

Clinical factors associated with late seizure remission after failed epilepsy surgery.

Epilepsy & behavior : E&B·2022

Same author

Antiepileptic action of c-Jun N-terminal kinase (JNK) inhibition in an animal model of temporal lobe epilepsy.

Neuroscience·2017

Same journal

Simultaneous Ca<sup>2+</sup> Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices.

Current protocols in neuroscience·2020

Same journal

Automated Two-Chamber Operon ID/ED Task for Mice.

Current protocols in neuroscience·2020

Same journal

A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.

Current protocols in neuroscience·2020

Same journal

Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.

Current protocols in neuroscience·2020

Same journal

Recombinant Antibodies in Basic Neuroscience Research.

Current protocols in neuroscience·2020

Same journal

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.

Current protocols in neuroscience·2020

See all related articles

Recording from neuronal dendrites is now possible thanks to advanced electrophysiological techniques. These methods utilize infrared optics and high-resolution micromanipulators for precise pipet positioning in unstained brain slices.

Area of Science:

Neuroscience
Cellular Biology

Background:

Pyramidal neurons possess complex apical dendrites crucial for neuronal signaling.
Understanding dendritic function requires advanced electrophysiological recording methods.

Purpose of the Study:

To describe the procedures for advanced electrophysiological techniques used for recording from neuronal dendrites.
To enable researchers to study the signaling properties of neuronal dendrites.

Main Methods:

Utilizing infrared optics for visualizing dendritic processes in unstained brain slices.
Employing high-resolution micromanipulators for precise pipet positioning.
Implementing stringent brain slice preparation techniques to maintain dendritic health in mature animal tissue.

Main Results:

Related Experiment Videos

Successful visualization of dendritic processes in unstained brain slices.
Accurate electrophysiological recordings from neuronal dendrites achieved.
Preservation of healthy dendritic processes in tissue from mature animals.

Conclusions:

Advanced electrophysiological techniques provide a viable method for recording from neuronal dendrites.
These techniques are essential for investigating the role of dendrites in neuronal signaling.
The described procedures facilitate research on neuronal function in the central nervous system.