Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Published on: June 26, 2013

Retrograde axonal tracing with fluorescent markers.

Brett R Schofield¹

¹Northeastern Ohio Universities College of Medicine, Rootstown, Ohio, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Circuit architecture and axonal branching in the efferent auditory system.

Hearing research·2026

Same author

Ultrastructural characterization of neuropeptide Y synapses in the central inferior colliculus of the Fischer Brown Norway rat.

Neuropeptides·2025

Same author

The Ventral Tectal Longitudinal Column: A Midbrain Nucleus for Modulation of Auditory Processing in the Cochlear Nucleus, Superior Olivary Complex, and Inferior Colliculus.

The Journal of comparative neurology·2025

Same author

The Ventral Tectal Longitudinal Column: A Midbrain Nucleus for Modulation of Auditory Processing in the Cochlear Nucleus, Superior Olivary Complex and Inferior Colliculus.

bioRxiv : the preprint server for biology·2025

Same author

Expression of group II mGluRs in the inferior colliculus, medial geniculate body, and auditory cortex increases with age.

Neuroscience·2024

Same author

Lineage-tracing reveals an expanded population of NPY neurons in the inferior colliculus.

Journal of neurophysiology·2024

Same journal

Simultaneous Ca<sup>2+</sup> Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices.

Current protocols in neuroscience·2020

Same journal

Automated Two-Chamber Operon ID/ED Task for Mice.

Current protocols in neuroscience·2020

Same journal

A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.

Current protocols in neuroscience·2020

Same journal

Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.

Current protocols in neuroscience·2020

Same journal

Recombinant Antibodies in Basic Neuroscience Research.

Current protocols in neuroscience·2020

Same journal

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.

Current protocols in neuroscience·2020

See all related articles

This study details six fluorescent tracers for neuronal circuit analysis. It guides researchers in selecting the best tracer and injection method for their specific neuroscience experiments.

Area of Science:

Neuroscience
Cell Biology
Biotechnology

Background:

Advancements in fluorescence imaging and sensitive fluorescent retrograde tracers offer new ways to study neuronal circuits.
Fluorescent markers enable combining axonal tract tracing with techniques like immunohistochemistry and physiological recording.

Purpose of the Study:

To provide guidance on selecting and using fluorescent tracers for neuronal circuit analysis.
To describe protocols for the application of various fluorescent tracers and injection methods.

Main Methods:

Description of six fluorescent tracers: Fast Blue, fluorescein dextran, FluoroGold, FluoroRuby, red beads, and green beads.
Detailed methods for tracer injection: pressure injection (microsyringe, micropipet) and iontophoretic injection (micropipet).

More Related Videos

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 12, 2020

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Published on: April 19, 2012

Related Experiment Videos

Last Updated: Jul 5, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Published on: June 26, 2013

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 12, 2020

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Published on: April 19, 2012

Discussion of criteria for choosing appropriate tracers and injection techniques.

Main Results:

Provides a comprehensive overview of available fluorescent tracers for axonal tracing.
Offers practical protocols for implementing these tracers in experimental settings.
Enables researchers to effectively analyze neuronal circuits using fluorescence imaging.

Conclusions:

The described protocols empower researchers to utilize a wide range of fluorescent tracers for diverse scientific questions in neuroscience.
Effective selection and application of fluorescent tracers are crucial for advancing the understanding of neuronal circuits.