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Updated: Jul 5, 2026

Chemical Triphosphorylation of Oligonucleotides

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Published on: June 2, 2022

Cartridge methods for oligonucleotide purification.

A Andrus¹, R G Kuimelis

¹PE Applied Biosystems, Foster, California, USA.

Current Protocols in Nucleic Acid Chemistry

|April 23, 2008

Summary

This study details protocols for purifying oligonucleotides using dimethoxytrityl-sensitive and affinity desalting methods. These methods leverage disposable solid-phase media for efficient and rapid purification.

Area of Science:

Biochemistry
Molecular Biology
Chemical Synthesis

Background:

Oligonucleotide purification is crucial for molecular biology applications.
Existing methods can be time-consuming and resource-intensive.

Purpose of the Study:

To provide standardized protocols for oligonucleotide purification.
To highlight the utility of disposable purification products.

Main Methods:

Dimethoxytrityl-sensitive purification.
Affinity desalting techniques.
Utilizing prepackaged, single-use cartridges and columns.

Main Results:

Developed efficient protocols applicable to various disposable purification products.

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Demonstrated successful purification using selective adsorption and elution on solid-phase media.

Conclusions:

The described protocols offer a rapid and convenient approach to oligonucleotide purification.
Disposable solid-phase media facilitate streamlined workflows in molecular biology and chemical synthesis.