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Updated: Jul 5, 2026

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Published on: March 5, 2018
Use of electrophilic substitution to form site-specific cross-links in DNA
1The Ohio State University, Columbus, Ohio, USA.
Abstract:
This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4-thio-2'-deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis-electrophilic tethers to form site-specific cross-links with a complementary DNA target. These modified oligodeoxynucleotides provide a flexible tool for studies directed at biophysical investigation of oligonucleotide structure, as well as investigation of DNA hybridization, stability, and sequence-dependent base nucleophilicity.
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