Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selection for catalytic function with nucleic acids.

R R Breaker1

  • 1Yale University, New Haven, Connecticut, USA.

Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New insight on the response of bacteria to fluoride.

Caries research·2012
Same author

Improved genetic transformation methods for the model alkaliphile Bacillus halodurans C-125.

Letters in applied microbiology·2011
Same author

Engineering ligand-responsive gene-control elements: lessons learned from natural riboswitches.

Gene therapy·2009
Same author

Gene expression control: harnessing RNA switches.

Gene therapy·2009
Same author

In vitro selection and characterization of cellulose-binding RNA aptamers using isothermal amplification.

Nucleosides, nucleotides & nucleic acids·2008
Same author

Riboswitches in eubacteria sense the second messenger cyclic di-GMP.

Science (New York, N.Y.)·2008
Same journal

Synthesis of Unmodified Oligonucleotides.

Current protocols in nucleic acid chemistry·2022
Same journal

Biophysical Analysis of Nucleic Acids.

Current protocols in nucleic acid chemistry·2022
Same journal

RNA Folding Pathways.

Current protocols in nucleic acid chemistry·2022
Same journal

Nucleic Acid Binding Molecules.

Current protocols in nucleic acid chemistry·2022
Same journal

Biologically Active Nucleosides.

Current protocols in nucleic acid chemistry·2020
Same journal

Biologically Active Nucleosides.

Current protocols in nucleic acid chemistry·2020
See all related articles

Designing effective in vitro selection protocols is key for discovering catalytic polynucleotides. This guide offers principles and examples for creating new selection strategies to accelerate the discovery of functional nucleic acids.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Synthetic Biology

Background:

  • In vitro selection is a powerful method for discovering novel nucleic acid catalysts (ribozymes and DNAzymes).
  • Designing effective selection protocols is crucial for efficiently isolating polynucleotides with desired catalytic functions.
  • Existing protocols often require specific strategies to couple catalytic activity with the amplification process.

Purpose of the Study:

  • To provide guidelines for the design of in vitro selection experiments targeting catalytic polynucleotides.
  • To present representative protocols that exemplify successful selection strategies.
  • To establish a conceptual framework for developing new selective-amplification protocols for nucleic acids.

Main Methods:

  • The unit outlines general principles for designing in vitro selection experiments.

Related Experiment Videos

  • It details several established protocols for selecting catalytic nucleic acids.
  • The focus is on strategies that link catalytic activity to the selection process.

    • Main Results:

    • The provided guidelines facilitate the rational design of selection experiments.
    • Representative protocols demonstrate successful isolation of functional catalytic polynucleotides.
    • A conceptual basis is established for creating novel selection systems.

    Conclusions:

    • Effective design of in vitro selection protocols is essential for advancing the field of catalytic nucleic acids.
    • The principles and examples presented can guide researchers in developing new and improved selection strategies.
    • This work supports the ongoing discovery and engineering of functional polynucleotides.