Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

Downstream Processing

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biospecific affinity elution.

Methods in molecular biology (Clifton, N.J.)·2011

Same author

Overview of protein purification and characterization.

Current protocols in protein science·2008

Same author

Protein purification flow charts.

Current protocols in protein science·2008

Same author

Saccharococcus caldoxylosilyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium.

International journal of systematic and evolutionary microbiology·2000

Same author

Structure of the IgG-binding ligand of the T-gel.

Journal of molecular recognition : JMR·1999

Same author

Longer is better--random elongation mutagenesis.

Nature biotechnology·1999

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Strategies for protein purification.

R K Scopes¹

¹La Trobe University, Bundoora, Australia.

Current Protocols in Protein Science

|April 23, 2008

Summary

This summary is machine-generated.

This guide details protein classification by shape, structure, and function. It outlines purification strategies for various protein types, including recombinant proteins, based on cellular location and solubility.

More Related Videos

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

Published on: September 29, 2023

Related Experiment Videos

Last Updated: Jul 5, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

Published on: September 29, 2023

Area of Science:

Biochemistry
Molecular Biology
Protein Chemistry

Background:

Proteins are classified based on their physical characteristics and biological roles.
Understanding protein properties is crucial for effective purification.
Cellular localization and solubility significantly impact protein purification strategies.

Purpose of the Study:

To provide a comprehensive overview of protein classification.
To discuss diverse strategies for purifying proteins based on their characteristics and cellular origins.
To detail methods for purifying recombinant proteins, including those forming inclusion bodies.

Main Methods:

Classification of proteins by shape, oligomeric structure, and function.
Discussion of purification techniques tailored to protein location (extracellular, cytoplasmic, membrane-associated).
Strategies for purifying insoluble proteins and recombinant proteins (soluble and inclusion bodies).

Main Results:

Proteins can be systematically classified using structural and functional criteria.
Purification methods must be adapted to the specific properties and location of target proteins.
Effective protocols exist for purifying both soluble and insoluble recombinant proteins.

Conclusions:

A structured approach to protein classification aids in selecting appropriate purification methods.
Tailored purification strategies are essential for isolating proteins from complex cellular environments.
Successful purification of recombinant proteins, regardless of solubility, is achievable with optimized techniques.