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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
09:27

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Published on: January 17, 2014

Generation of recombinant vaccinia viruses

P L Earl1, B Moss, L S Wyatt

  • 1National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed

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Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...