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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Related Experiment Video

Updated: Jul 5, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Analytical ultracentrifugation.

¹University of New Hampshire, Durham, New Hampshire, USA.

Current Protocols in Protein Science

|April 23, 2008

Summary

This summary is machine-generated.

Analytical ultracentrifugation is a powerful technique in molecular biology and biochemistry. This overview covers its applications and essential experimental design considerations for researchers.

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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Published on: November 5, 2009

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Area of Science:

Biochemistry
Molecular Biology

Background:

Analytical ultracentrifugation (AUC) is a powerful biophysical technique.
Its potential in molecular biology and biochemistry remains underexploited.

Purpose of the Study:

To provide an overview of AUC applications.
To highlight key considerations for experimental design in AUC.

Main Methods:

This study is an overview, not experimental.
It focuses on describing the applications and design principles of AUC.

Main Results:

AUC offers diverse applications in biological and biochemical research.
Proper experimental design is crucial for successful AUC.

Conclusions:

Analytical ultracentrifugation is a valuable tool for biological and biochemical studies.
Understanding its applications and design is key to maximizing its utility.