A three-fragment phenotype in the D16S309 locus

An allele with internal restriction site or another kind of mutation has been found in D16S309 locus. Using a probe MS205 in combination with the enzyme Hinf I 3-fragment, a pattern was observed in a woman from the Upper Silesia population--the mother of a child involved in paternity testing. DNA from this woman was additionally digested with Alu I and Mbo I enzymes. All the digested samples showed a 3-fragment pattern with the probe MS205, while other probe/enzyme combinations detected only the expected phenotypes. The disputed child inherited a second, normal allele from its mother.