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Updated: Jul 5, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber
Kwang Ho Cheong1, Dong Kee Yi, Jeong-Gun Lee
1Bio & Health Lab, Samsung Advanced Institute of Technology, Giheung, Korea. kwangho.cheong@samsung.com
Abstract:
The optothermal properties of nanoparticles are of interest for biosensors and highly sensitive biochip applications. In this respect, the longitudinal resonance of Au nanorods was used to transform near infrared energy into thermal energy in a microfluidic chip. The resulting heat generated effectively caused pathogen lysis. Consequently the DNA was extracted out of the cell body and transferred to a PCR system. This resulted in the successful demonstration of a one step real-time PCR system for pathogen detection without removal or changing of reagents.

