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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Use of monoclonal antibodies for expression cloning

D Hollenbaugh1, A Aruffo, B Jones

  • 1Bristol-Myers Squibb, Seattle, Washington, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed

This unit details the use of transient expression in mammalian cells to screen cDNA libraries with monoclonal antibodies (MAb) to isolate cDNA clones encoding cell-surface and intracellular proteins. The first protocol describes the cloning of cDNAs encoding cell-surface antigens. The second protocol is a modification that facilitates isolation of cDNAs encoding antigens that are expressed intracellularly. Both protocols are designed for use with the expression vector CDM8, which contains a polylinker for subcloning double-stranded cDNA.

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