Related Experiment Video
Updated: Jul 5, 2026
04:23
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Published on: April 28, 2019
Animal models using lymphocytic choriomeningitis virus
1The Scripps Research Institute, La Jolla, California, USA.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details methods for infecting mice with lymphocytic choriomeningitis virus (LCMV) and measuring immune responses. Protocols cover T cell and antibody detection, aiding LCMV research.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Lymphocytic choriomeningitis virus (LCMV) is a model pathogen for studying viral infections and immune responses.
- Understanding host-pathogen interactions requires robust methods for infection and immune assessment.
Purpose of the Study:
- To provide comprehensive protocols for inducing and maintaining LCMV infection in mice.
- To describe methods for quantifying T cell and antibody responses against LCMV.
- To offer essential support protocols for LCMV stock preparation and titer measurement.
Main Methods:
- Induction of systemic and persistent LCMV infection in murine models.
- Measurement of T cell responses specific to LCMV.
- In vivo assessment of anti-LCMV immunity.
- LCMV stock preparation and plaque assays for viral titration.
- Enzyme-linked immunosorbent assay (ELISA) for anti-LCMV antibody detection.
Main Results:
- Established protocols enable reliable induction of LCMV infection in mice.
- Methods for measuring T cell and antibody responses are detailed.
- Support protocols facilitate LCMV stock management and quantification.
Conclusions:
- The provided protocols offer a standardized framework for LCMV research.
- These methods are crucial for investigating viral pathogenesis and immune control of LCMV.
