Related Experiment Video
Updated: Jul 5, 2026
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
Published on: March 1, 2011
Measurement of interleukin 6
R P Nordan1, C D Richards, J Gauldie
1National Cancer Institute, Bethesda, Maryland, USA.
This study presents a sensitive bioassay for quantifying Interleukin 6 (IL-6) across multiple species by measuring cell proliferation. This method offers a viable alternative to ELISA kits for assessing IL-6 bioactivity.
Area of Science:
- Immunology
- Cell Biology
Background:
- Interleukin 6 (IL-6) is a key cytokine involved in immune responses and cellular processes.
- IL-6 is produced by various cells upon stimulation, highlighting its broad biological significance.
Purpose of the Study:
- To describe a simple and sensitive bioassay for quantifying Interleukin 6 (IL-6) in multiple species.
- To provide protocols for maintaining cell lines and preparing reagents for IL-6 measurement.
Main Methods:
- Utilized an IL-6-dependent murine B cell hybridoma cell line (B9) to assess IL-6 bioactivity.
- Developed protocols for B9 cell maintenance, IL-6 standard preparation, and supernatant collection.
Main Results:
- Established a sensitive assay for human, rat, rabbit, pig, and mouse IL-6.
- Demonstrated the utility of the B9 cell proliferation assay for IL-6 quantification.
Conclusions:
- The described bioassay offers a sensitive and species-versatile method for measuring IL-6 bioactivity.
- ELISA kits are an alternative for human/mouse IL-6 measurement but do not assess bioactivity.
More Related Videos
09:43Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
Published on: March 8, 2024
08:26Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018