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Updated: Jul 5, 2026
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
Single tube optimisation of APOE genotyping based on melting curve analysis
Francesc Francés1, Olga Portolés, José Vicente Sorlí
1Genetic and Molecular Epidemiology Unit, Department of Preventive Medicine, School of Medicine. University of Valencia, Valencia, Spain. francesc.frances@uv.es
Objectives:
To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes.
Design And Methods:
We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions.
Results:
Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol.
Conclusions:
This method is cheaper, faster and more reliable and versatile than other alternatives proposed.
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