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Updated: Jul 5, 2026

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Early epithelial phenotypic changes predict graft fibrosis.

Alexandre Hertig1, Dany Anglicheau, Jérôme Verine

  • 1Urgences Néphrologiques and Transplantation Rénale, Hôpital Tenon, 4 rue de la Chine, 75020 Paris, France. alexandre.hertig@tnn.aphp.fr

Journal of the American Society of Nephrology : JASN
|April 25, 2008
PubMed
Summary
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Early detection of specific cellular changes in kidney allografts can predict fibrosis progression. Identifying these markers in tubular epithelial cells offers a potential biomarker for chronic allograft nephropathy.

Area of Science:

  • Nephrology
  • Transplantation Immunology
  • Cell Biology

Background:

  • Chronic allograft nephropathy leads to significant kidney allograft loss.
  • Early detection of interstitial fibrosis and tubular atrophy is crucial for timely intervention.
  • No reliable biomarker currently exists for early detection of these changes.

Purpose of the Study:

  • To investigate whether phenotypic changes in tubular epithelial cells, indicative of epithelial-to-mesenchymal transition, can predict fibrosis progression in renal allografts.
  • To assess the correlation between early markers of epithelial-to-mesenchymal transition and interstitial fibrosis development.

Main Methods:

  • Eighty-three kidney transplant recipients underwent protocol biopsies at 3 and 12 months post-transplantation.
  • Immunohistochemistry was used to detect de novo vimentin expression and beta-catenin translocation in tubular cells at 3 months.

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Related Experiment Videos

Last Updated: Jul 5, 2026

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
07:51

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

Published on: January 12, 2019

  • Interstitial fibrosis scores were assessed at 1 year and changes between 3 and 12 months were analyzed.

    • Main Results:

    • Expression of vimentin and beta-catenin translocation in >=10% of tubules at 3 months was associated with higher interstitial fibrosis scores at 1 year.
    • The intensity of these phenotypic changes positively correlated with fibrosis progression.
    • These early changes were identified as independent risk factors for fibrosis progression and were linked to poorer graft function at 18 months.

    Conclusions:

    • Early phenotypic changes in tubular epithelial cells, suggestive of epithelial-to-mesenchymal transition, serve as predictive markers for interstitial fibrosis progression in human renal allografts.
    • These findings may lead to the development of novel biomarkers for early diagnosis and intervention in chronic allograft nephropathy.