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A case of pulmonary cryptococcosis in a free-living toad (Bufo bufo)
Fernanda Seixas1, Maria da Luz Martins, Maria de Lurdes Pinto
1Laboratório de Histologia e Anatomia Patológica, Clínicas Veterinárias, Universidade de Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
Abstract:
Pulmonary cryptococcosis was observed in a free-living adult female common toad (Bufo bufo) that was killed by a vehicle. Both lungs had various eosinophilic, monomorphic, and spherical to elliptical organisms identified as Cryptoccocus spp. The yeasts were demonstrated by Grocott's silver method and the periodic acid-Schiff reaction and the capsule was positive for mucin with a mucicarmine stain. The agent was confirmed by immunohistochemistry, using the monoclonal antibody anti-Cryptococcus neoformans, and by a polymerase chain reaction-based method using a C. neoformans-specific primer. This report, to the best of our knowledge, represents the first case of cryptococcosis in a common toad.
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