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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
Published on: May 26, 2021
Chromosomal instability in rhesus macaque preimplantation embryos
Cathérine Dupont1, Lutz Froenicke, Leslie A Lyons
1Departments of Obstetrics & Gynecology and Physiology, CS Mott Center for Human Growth and Development, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA. cdupont@med.wayne.edu
Fertility and Sterility
|April 29, 2008
Summary
Rhesus macaques provide a valuable animal model for studying chromosomal instability in human embryos. Their preimplantation embryos show similar numerical chromosomal aberrations, aiding research into human reproductive health.
Area of Science:
- Reproductive Biology
- Genetics
- Developmental Biology
Background:
- Chromosomal instability is a significant factor in human oocyte and preimplantation embryo development.
- Identifying a suitable animal model is crucial for investigating the origins of this instability.
Purpose of the Study:
- To establish a relevant animal model for systematically investigating chromosomal instability in human oocytes and preimplantation embryos.
- To assess the utility of rhesus macaques for this research.
Main Methods:
- Prospective in vitro fertilization (IVF) study using young rhesus macaque females.
- Cytogenetic assessment of preimplantation embryos using a five-color fluorescent in situ hybridization (FISH) assay.
- FISH assay targeted chromosomes homologous to human chromosomes 13, 16, 18, X, and Y.
Main Results:
- Fifty preimplantation embryos from 11 rhesus macaques were analyzed.
- Chromosomal abnormalities were observed in 46% of embryos.
- Specific abnormalities included mosaic (22%), chaotic (6%), aneuploid (4%), haploid (6%), and triploid (8%) embryos.
Conclusions:
- In vitro produced rhesus macaque and human preimplantation embryos display comparable numerical chromosomal aberrations.
- Rhesus macaques represent a suitable animal model for studying the origins of chromosomal instability in human preimplantation embryos.
