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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Cryptosporidium sp in 300 children with and without diarrhea

E García Velarde1, M Chávez Legaspi, P Coello Ramírez

  • 1Hospital de Pediatria, Centro Medico de Occidente, IMSS.

Archivos De Investigacion Medica
|July 1, 1991
PubMed

Insights

Cryptosporidium infection was detected in 3.6% of stool samples. Malnourished children under one year with diarrhea showed a higher frequency of Cryptosporidium.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Pediatrics

Background:

  • Cryptosporidium is an opportunistic protozoan parasite.
  • Diarrhea is a common symptom of Cryptosporidium infection, particularly in children.

Purpose of the Study:

  • To investigate the prevalence of Cryptosporidium in patients with diarrhea and other diseases.
  • To identify risk factors associated with Cryptosporidium infection.

Main Methods:

  • Prospective study of 403 stool samples over six months.
  • Ziehl-Nielsen acid-fast staining technique used for Cryptosporidium detection.

Main Results:

  • Cryptosporidium was identified in 3.6% of samples (11/304).
  • 81% of positive cases (9/11) were from patients with diarrhea.
  • Oocysts were found in 5% of malnourished children (II or III) with diarrhea.
  • 91% of positive cases (10/11) were in children under one year of age.
  • A statistically significant increase in Cryptosporidium frequency was observed in malnourished children with diarrhea (p < 0.05).

Conclusions:

  • Cryptosporidium is a relevant cause of diarrhea in children, especially those who are malnourished and under one year old.
  • Early detection and intervention are crucial for managing Cryptosporidium infections in vulnerable pediatric populations.

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