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"Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA

Y F Lee, E Wimmer

    Nucleic Acids Research
    |July 1, 1976
    PubMed

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    Two-dimensional Gel Electrophoresis01:22

    Two-dimensional Gel Electrophoresis

    Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
    The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
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