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Updated: Jul 5, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Repeatability of monolithic HPLC columns while using a flow program.

Lukas Kaminski¹, Sami El Deeb, Hermann Wätzig

¹Institute of Pharmaceutical Chemistry, Technical University of Braunschweig, Braunschweig, Germany.

Journal of Separation Science

|May 1, 2008

Summary

Fast High-Performance Liquid Chromatography (HPLC) methods using monolithic columns can be accelerated with flow programming. While high flow rates may slightly impact precision, appropriate equipment ensures flow programming is a viable technique for reducing run times without compromising repeatability.

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Area of Science:

Analytical Chemistry
Chromatography

Background:

Fast High-Performance Liquid Chromatography (HPLC) is increasingly vital for efficient analysis.
Monolithic HPLC columns enable rapid separations, with flow programming offering further time reduction.

Purpose of the Study:

To evaluate the impact of flow programming on the repeatability of fast HPLC separations.
To assess the reliability of a generic assay for glibenclamide and glimepiride using flow programming.

Main Methods:

Utilized monolithic HPLC columns for fast separations.
Implemented a flow program from 5.0 to 9.9 mL/min to reduce run time to approximately 1.7 minutes.
Investigated the repeatability of retention times and peak areas for glibenclamide and glimepiride and their degradation products.

Main Results:

Within-day repeatability (RSD% < 1%) was excellent for retention times and peak areas.
Day-to-day precision (RSD% up to 2%) showed some impairment at flow rates exceeding 7 mL/min.
Impaired repeatability was attributed to pump inefficiency at high flow rates, not the flow program itself.

Conclusions:

Flow programming is a reasonable strategy for significantly shortening HPLC run times.
With appropriate high-flow-rate equipment, flow programming does not negatively affect analytical method repeatability.
Fast HPLC methods utilizing flow programming can be reliably applied in pharmaceutical analysis.